Saltwater Testing Methods
Whole Effluent Toxicity (WET) tests were developed as a tool to evaluate the potential harmful effects of effluent discharged into surface water. Carefully chosen indicator organisms are exposed to whole effluent and/or effluent dilutions for a specified period of time to observe the effluent's effect on the organisms and its potential to effect organisms in the receiving stream. Test methods and duration of the tests are specified in the discharger's permit.
Whether you are a waste water treatment plant, chemical plant, refinery, or product manufacturer, contact us to discuss your permit or research needs.
EPA - 821-R-02-012, Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms.
24, 48 & 96 Hour Definitive Tests:
Method 2007, Americamysis bahia (formerly Mysidopsis bahia), Mysid shrimp
Method 2006, Menidia beryllina, Inland Silverside Minnow
Americamysis bahia
Americamysis bahia
EPA - 821-R-02-014, Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms
7-Day Chronic Toxicity Tests:
Method 1007, Survial, Growth, and Fecundity Test Americamysis bahia
Menida beryllina
Method 1006, Survial and Growth Test, Menidia beryllina
LC50, IC25, and NOEC Statistical Analyses Available
All organisms for primary test methods are cultured in-house, providing immediate availability as well as exceptional quality organisms.
EE USA's staff are thoroughly and annually trained to perform tests according to Standard Operating Procedures developed following EPA methodology and NELAP standards. They have the skills and experience to develop methods for special projects as well as complete primary test methods.